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. 2012 Jul 9;7(7):e39604. doi: 10.1371/journal.pone.0039604

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Relevant characteristicsa Reference or source
P. syringae pv. aesculi
PD4818 A. hippocastanum isolate, Winssen, the Netherlands, 2004, CbR Plantenziektenkundige Dienst, Wageningen, the Netherlands
PD5126 A. hippocastanum isolate, the Netherlands, 2005, CbR Plant Research International, Wageningen, the Netherlands
E. coli
DH5α F, φ80ΔlacZM15, recA1, endA1, hsdR17, phoA, supE44, gyrA96,relA1, λ [48]
GM119 F, dam , dcm , metB1, lacY1, galK2, galT22, tonA31, tsx-78,supE44, mtl-1 Dr. M. G. Marinus (University of Massachusetts medical school)
Cel40 Tra+ (pRK2013), KmR E. L. Lagendijk (Molecular Microbiology Leiden University)
plasmids
pMP4655 oriBBR1, oriVS1, oriT (p15A), lac::eGPF, TcR [18], [19]
pRK2013 oriColE1, Tra (RK2), KmR [49]
a

Abbreviations used: Cb, carbenicillin; Km, Kanamycin; Tc, Tetracycline; Tra, conjugal transfer functions.