Table 1. Bacterial strains and plasmids used in this study.
Strain or plasmid | Relevant characteristicsa | Reference or source |
P. syringae pv. aesculi | ||
PD4818 | A. hippocastanum isolate, Winssen, the Netherlands, 2004, CbR | Plantenziektenkundige Dienst, Wageningen, the Netherlands |
PD5126 | A. hippocastanum isolate, the Netherlands, 2005, CbR | Plant Research International, Wageningen, the Netherlands |
E. coli | ||
DH5α | F−, φ80ΔlacZM15, recA1, endA1, hsdR17, phoA, supE44, gyrA96,relA1, λ− | [48] |
GM119 | F−, dam −, dcm −, metB1, lacY1, galK2, galT22, tonA31, tsx-78,supE44, mtl-1 | Dr. M. G. Marinus (University of Massachusetts medical school) |
Cel40 | Tra+ (pRK2013), KmR | E. L. Lagendijk (Molecular Microbiology Leiden University) |
plasmids | ||
pMP4655 | oriBBR1, oriVS1, oriT (p15A), lac::eGPF, TcR | [18], [19] |
pRK2013 | oriColE1, Tra (RK2), KmR | [49] |
Abbreviations used: Cb, carbenicillin; Km, Kanamycin; Tc, Tetracycline; Tra, conjugal transfer functions.