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. 2012 Jul 9;7(7):e38701. doi: 10.1371/journal.pone.0038701

Figure 4. Accumulation rate of gentisate by C. glutamicum RES167ΔgenK expressing wild-type and mutant GenK proteins.

Figure 4

Proteins were expressed in C. glutamicum RES167 ΔgenK cells from the E. coli-C. glutamicum shuttle vector pXMJ19 constructs. All strains were grown in LB with gentisate and IPTG induction. The rate of accumulation of gentisate by strain RES167 ΔgenK [pXMJ19-genK] containing wild-type GenK protein (2.47±0.23 nmol/min/mg of dry weight) was set as 100%. Wild Type, wild-type GenK in strain RES167 ΔgenK [pXMJ19-genK]; D54E, mutant GenK of Asp-54 substituted with glutamate in strain RES167 ΔgenK [pZWXYCg02]; D57E, mutant GenK of Asp-57 substituted with glutamate in strain RES167 ΔgenK [pZWXYCg04]. All assays were performed in triplicate and standard deviations are represented by error bars.