Figure 6. Substrate inhibition of GenK-mediated gentisate uptake in C. glutamicum RES167.

The concentrations of gentisate and competing substrate were 40 µM and 800 µM, respectively. The rate of accumulation of gentisate (without competing substrates) was 2.19±0.14 nmol/min/mg of cell dry weight, which was set as 100%. Inhibition was determined by comparing the rate of gentisate uptake in the absence and presence of competing substrate. Values are the averages from three experiments and standard deviations are represented by error bars.