Table 3. An analysis of experimentally observed secondary structures in prokaryotic glycosites.
| Protein Name (Source organism) | PDB ID | Presence of glycanin structure | Validated Glycositesin full length protein sequence | Position of Glycositesin PDB entry sequence | SS |
| N-Glycosylated Proteins | |||||
| Tetrabrachion(Staphylothermus marinus) | 1YBK,1FE6 | – | N44, N605, N641,N685, N708, N1279,N1402 | N44 (N1279*) | H1 |
| Chondroitinase ABC(Proteus vulgaris) | 1HN0 | – | N282, N338, N345,N515, N675, N856,N963 | N282, N963 & N675N338, N345 & N515N856 | FR1 H1 B1 |
| PotD (Escherichia coli) | 1POT,1POY | – | N26, N62 | N26N62 | FR3 FR1 (at beginning of helix) |
| AcrA (Campylobacter jejuni) | 2K32,2K33(NMR) | Heptasaccharide | N123, N273 | N42 (N123*) | FR1 |
| PEB3 (Campylobacter jejuni) | 2HXW | - | N90 | N90 | FR1 (between helices) |
| HmcA (Desulfovibrio gigas) | 1Z1N | Trisaccharide (NAG,NAA,any epimer of NAG), | N290 | N261 | FR2 (between beta-sheets) |
| O-Glycosylated Proteins | |||||
| Chondroitinase-AC(Pedobacter heparinus) | 1CB8,1HM2,1HM3,1HMU,1HMW | Tetrasaccharide Man-(Rha)-GlcUA-Xyl, | S328, S455 | S328 S455 | FR1 (just after helix) FR1 (between beta-sheets) |
| Chondroitinase-B(Pedobacter heparinus) | 1DBG,1DBO,1OFL,1OFM | Heptasaccharide galactose-β(1–4)[galactose-α(1–3)](2-O-Me)fucose-β(1–4)xylose-β (1–4)glucuronicacid-α(1–2)[rhamnose-α(1–4)]mannose-α(1- | S234 | S234 | FR1 (between beta-strands) |
| Heparinase II(Pedobacter heparinus) | 2FUQ,2FUT | Tetrasaccharide Man-(Rha)-GlcUA-Xyl (xylose-β(1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1- | T134 | T134 | H3 |
| Fimbrial protein(Neisseria gonorrhoeae) | 2HI2,2HIL,2PIL,1AY2 | Disaccharides α-D-galactopyranosyl-(1→3)-2,4-diacetamido-2,4-dideoxy-β-D-glucopyranoside(bacillosamine, Bac);Gal-DADDGlc; andGlcNAc-α1,3-Gal | S70 | S63 | H1 (before helix) |
| Glycocin F(Lactobacillus plantarum) | 2KUY(NMR) | Two N-Acetylglucosamines | S39 | S18 | FR3 |
| Endo-β-N-acetylglucosaminidase F3 (Flavobacterium meningosepticum) | 1EOM,1EOK | - | T88 | T49 | FR1 |
Footnotes: All crystal structures are obtained from www.rcsb.org. All structures are at a resolution of 1.4 Å or above.
Symbols used: - : No sugar detected, *: Corresponding position in full length protein sequence, F: flexible Regions with turns/loops/coils/bends or no assigned secondary structure, H: helix, B: beta sheet, 1: Intra domain, 2: Interdomain, 3: no assigned domain.