Fig. 8. CB2 inhibits neurotransmission through inhibition of voltage gated calcium channels.
(A,B) In CB2 expressing CB1 null neurons, exchanging the external medium with medium containing 0.2 mM CaCl2 (low Ca2+) increases the inter-event interval of sEPSCs (n=9). The low Ca2+ medium occludes the inverse agonist effect of AM630 observed in Fig. 7. (C) Exchanging the medium with low Ca2+ does not alter sEPSC amplitude. AM630 also has no further effect on sEPSC amplitude (n=9). Data in (B) and (C) analyzed using one-way ANOVA with Bonferroni's multiple comparison test.