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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 13;189(2):906–915. doi: 10.4049/jimmunol.1200257

Fig. 2. LTB4 is necessary for dectin-1 responses in macrophages.

Fig. 2

(A–C) WT and 5-LO−/− macrophages were pretreated ± LTB4 for 24 h, then incubated with the dectin-1 selective agonist curdlan (100 µg/ml) for another 24 h prior to determination of IL-12 p40 (A), TNF-α (B), and GM-CSF (C) levels by ELISA. (D–F) WT macrophages were incubated with the 5-LO inhibitor AA-861 (10 µM) or the BLT1 antagonist U7532 (1 µM) for 24 h, followed by curdlan stimulation for 24 h and IL-12 p40 (D), TNF-α (E), and GM-CSF (F) levels determined by ELISA. Data represent the mean ± SEM from 3 individual experiments, each performed in triplicate. *p < 0.05 versus WT control or untreated control; #p < 0.01 versus untreated 5-LO−/− macrophages or untreated WT and stimulated with curdlan by ANOVA.