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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 6;189(2):587–597. doi: 10.4049/jimmunol.1200840

Figure 5. Toso/FcμR does not mediate NK cytotoxicity.

Figure 5

A, top panel, NK cells were incubated with IgM anti-Lewis antigen Ab (10 μg/ml), then mixed with SK-OV3 target cells (left). SK-OV3 target cells were incubated with an IgG anti-HER2 mAb (50 ng/ml), then incubated with NK cells (right). Bottom panel, Flow cytometry illustrates the expression level of Toso/FcμR on NK cells (left) and binding of the IgM anti-Lewis Ab to SK-OV3 cells (right). B, top panel, P815 cells were pretreated with anti-Toso mAb (1 μg/ml; left) or anti-CD16 mAb (100 ng/ml; right) then incubated with NK cells. Bottom panel, Flow cytometry shows expression of Toso/FcμR by NK cells (left) and binding of anti-Toso mAb to P815 cells (right). C, top panel, anti-IgM mAb (1 μg/ml) coated P815 cells were incubated with NK cells in the presence or absence of mIgM (10μg/ml). Bottom panel, Flow cytometry demonstrates P815 cells are able to bind IgM-APC via anti-IgM mAb. The graphs display representative results and are consistent with data from an at least 3 donors.