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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Aspects Med. 2012 Apr 21;33(4):295–317. doi: 10.1016/j.mam.2012.04.005

Figure 6.

Figure 6

Serial sections of submacular choroid from normal aged control (left) and retina and submacular choroid from an AMD subject (right) incubated with TSP-1, endostatin, and PEDF antibodies. Right panels are high magnification photos of left panels. (A–D) Hematoxylin and eosin (H&E) staining show morphological features of retina and choroid like migration of RPE cells into retina in AMD (C,D). Pigment in immunostained sections was bleached from RPE and choroidal melanocytes. Immunostaining of CD-34 (E–H) is associated with the retinal and choroidal blood vessels including CC (arrow). In aged control choroid, TSP-1 immunoreactivity (I and J) is intense especially in BrMb (arrowhead). Both endostatin (M and N) and PEDF (Q and R) are prominent in RPE basal lamina, BrMb, and CC basement membrane and show similar pattern and intensity of immunostaining. In contrast, expression of TSP-1 (K and L), endostatin (O and P), and PEDF (S and T) is greatly reduced in AMD choroid compared to the aged control and the reaction product of endostatin and PEDF appears more diffuse in choroidal stroma. (arrowhead, Bruch’s membrane; arrow, choriocapillaris).