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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 8;189(2):867–875. doi: 10.4049/jimmunol.1200465

Figure 5.

Figure 5

Impaired phagocytosis, bacterial killing, and reactive oxygen intermediate production in AMs from l/l mice. AMs obtained from WT (open bars) and l/l (solid bars) mice were assessed for their ability to phagocytose (A) and kill ingested K. pneumoniae opsonized with immune serum (B) according to the procedures described in the Materials and Methods section. *P < 0.05 compared to WT using a student t-test. Bars represent the mean ± SEM of at least 3 separate experiments. AMs from WT (open circles) and l/l (closed circles) mice were cultured with H2DCF for 1 h, and then stimulated with heat-killed K. pneumoniae opsonized with immune serum using a multiplicity of infection of 100:1 to assess reactive oxygen intermediate production (ROI) (C). ROI production was assessed using a fluorometer and expressed as relative fluorescence units. The data represent the mean of three experiments completed in quadruplicate for each time point using a two way analysis of variance. *P < 0.05 and ***P<0.001 compared WT.