Skip to main content
. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 18;189(2):793–803. doi: 10.4049/jimmunol.1200411

FIGURE 3.

FIGURE 3

Sle1c2 upregulates IFNγ expression. A, Representative histograms and quantification of ex vivo IFNγ intracellular staining in CD4+ T cells were from the spleens of 2–3 month old mice. Significance levels indicate ANOVA with Dunn's multiple comparison analysis to B6. B, Representative plots and quantitation of FoxP3+ IFNγ+ double positive CD4+ T cells after 3 d of culture with plate-bound anti-CD3/CD28 and TGFβ. Cells were collected from 1–3 and 8–10 month old mice. C, D, Representative plots (C) and quantification (D) of intracellular staining for IFNγ, FoxP3, and IL-17 in CD4+ T cells from 8–10 month old mice after 3 d of TH0 (anti-CD3 and anti-CD28 only), Treg (stimulation plus TGFβ), and TH17 (stimulation plus TGFβ and IL-6) polarizing conditions. In B and D, t tests compare B6 to Sle1c2 mice for each age or treatment group. : * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.