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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 18;189(2):793–803. doi: 10.4049/jimmunol.1200411

FIGURE 7.

FIGURE 7

Mitochondrial function is impaired in the CD4+ T cells of B6.Sle1c2 mice. A–E, Splenic CD3+ CD4+ T cells were assessed by flow cytometry for mitochondrial mass (A), mitochondrial membrane potential (B), oxidation (C), Ca (D) and NO (E) contents. The graphs show mean + SEM mean fluorescence intensity (MFI) normalized to B6 mean values. F, viability analysis of ex-vivo splenocytes based on Annexin V (Ann V) and PI staining. Live cells were Ann V− PI−. G, VDAC expression in CD4+ T cells analyzed by Western blot. β-actin expression is shown as a control. The graph on the right shows the densitometry analysis of the VDAC/ β-actin ratios normalized to one B6 sample. All data were collected from the same 2–3 months old mice. B6 and B6.Thy1a (B6 a) values were pooled in the graphs under B6 and compared to B6.Sle1c2 values with Mann-Whitney tests. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.