Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2012 Apr 24;11(7):1389–1399. doi: 10.1158/1535-7163.MCT-11-0887

Figure 5. Antagonizing PKD3 expression or activity leads to reduced secretion of IL-6, IL-8, and GROα.

Figure 5

A, Conditioned media from equal numbers shScr or shPKD3-1 C2-3 cells was subjected to ELISA to determine levels of secreted IL-6, IL-8, or GROα. B, PC3 and DU145 cells were transfected with siRNAs targeting different regions of PKD3 or non-targeting siRNA. Conditioned media from equal numbers of cells was then subjected to ELISA. C, PC3 cells were treated with the indicated inhibitors for 48 h. ELISA was performed on the conditioned media collected from equal numbers of cells. D, IL-6, IL-8, and GROα transcript levels relative to the GAPDH control were determined by RT-qPCR in the inducible cell lines. Each experiment was repeated with duplicates at least 2–3 times, and the data represent the mean ± S.E.M. of the independent experiments.