Table 1.
Characterization of dendrimer conjugates.
| Surface NH2 groups |
Fluorophore Attacheda |
Fluorophore Attachedb |
Measured MW (Da)c |
Theoretical MW (Da) |
ζ-Potential (mV)d | |
|---|---|---|---|---|---|---|
| G2-NH2 | 16 | 0 | 0 | 3,160-3,253 | 3,256 | +16.6 |
| G4-NH2 | 64 | 0 | 0 | 10,709-15,287 | 14,215 | +38.5 |
| G4-RITC-NH2 | 63 | 1.0 | - | 9,101-19,000 | 15,753 | +41.2 |
| G2-RITC-NH2 | 15 | 1.0 | 1.3 | 4,155 | 3,792 | +18.8 |
| G2-RITC-Ac | 0 | 1.0 | 1.3 | 4,399 | 4,398 | +2.7 |
| G2-RITC-COOH | 0 | 1.0 | 1.3 | 5,655 | 5,346 | −14.5 |
| G2-RITC-NH2-OA2.3 | 13 | 1.0 | 1.3 | 4,389 | 4,638 | N/Ae |
| G2-RITC-NH2-OA2.7 | 10 | 1.0 | 1.3 | 3,708-5,643 | 5,202 | N/Ae |
a
Measured using UV/Vis spectroscopy.
b
Measured using 1H NMR.
c
Meausred using MALDI-TOF.
d
Measured using Zeta Potential/Particle Sizer.
e
Zeta potential of OA conjugates were not measured due to the limited solubility in aqueous solutions.