Effect of heavy metal scavenger metallothionein (MT) on low temperature (LT, 4°C, 3 months)-induced change in intracellular Ca2+ homeostasis in cardiomyocytes. FVB and MT mice were kept at room temperature or 4°C for 3 months prior to intracellular Ca2+ assessment. A: Resting fura-2 fluorescence intensity (FFI); B: Electrically-stimulated rise in FFI (ΔFFI); C: Single exponential intracellular Ca2+ decay rate; and D: Bi exponential intracellular Ca2+ decay rate. Mean ± SEM, n = 59 – 60 cells from 4 mice per group, * p < 0.05, NT: normal temperature.