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. Author manuscript; available in PMC: 2013 Jul 6.
Published in final edited form as: Cell. 2012 Jun 28;150(1):39–52. doi: 10.1016/j.cell.2012.05.026

Figure 4. POT1b regulates overhangs at both sister telomeres.

Figure 4

(A, B) Overhang assay of asynchronous, G0 arrested, and released 1° POT1bF/F ROSA26-Cre-ERT2 MEFs with and without Cre induction (4-OHT). Values represent the mean of two experiments using independent POT1bF/F ROSA-Cre-ERT2 MEF lines and SEM. See legend of Fig. 1 for details. (C, D) Overhang analysis of POT1bF/− cells at 120 h post Cre in G1 and S/G2 isolated by FUCCI-FACS. (E, F) Overhang analysis of leading- and lagging-end telomeres from POT1bF/− MEFs at 120 h post-Cre (or withouit Cre). For slot blots of the CsCl gradient, see Fig. S5C. (G) Quantification of overhang analyses of POT1bF/− MEFs at 96 h following lentiviral shRNA to Stn1 and 120 h after Hit&Run Cre treatment. The signal in POT1bF/− MEFs without Cre is set to 1. Mean of 3 independent experiments and SDs. See also Figure S5.