Figure 6. POT1b inhibits hyper-resection by Apollo.
(A, B) Overhang analysis of ApolloF/F, POT1bF/F, and ApolloF/FPOT1bF/F MEFs without and at 120 h after Hit&Run Cre. See Fig. 1 for details. (C, D) Overhang analysis of ApolloF/FPOT1bF/F MEFs expressing the indicated POT1b alleles at 120 h after Cre. (E) Overhang analysis of the leading- and lagging-end telomeres from ApolloF/FPOT1bF/F MEFs in the absence of Cre and at 120 h post-Cre. (F) Relative overhang size in different genetic backgrounds. Values for Apollo/POT1b DKO cells are the mean and SDs of 3 independent experiments (see Figs. 2G, 3F and 6E for data on Apollo KO and POT1b KO). See also Figure S7.