Skip to main content
. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: J Mol Biol. 2012 May 7;421(1):30–37. doi: 10.1016/j.jmb.2012.04.032

Fig. 2. Verification of the location of H4 tails in the nucleosome by methyl-TROSY NMR and PRE.

Fig. 2

Methyl-TROSY spectra of the nucleosome with methyl-labeled H3 (C110A) and MTSL-labeled H4 at position A15 in (a) oxidized and (b) reduced form. (c) Plot showing changes in peak intensities for the methyl groups in H3 as a function of the distances between the Cβ atom of A15 in H4 and the carbon atoms in the methyl groups. Methyl groups that show large changes in peak intensities are labeled in the plot. A bar graph showing the peak intensity change is shown in the supplementary figure. (d) Structural illustration for the effects of PRE. The MTSL-labeling at position A15 of H4 follows the procedure of Racki et al.32