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Slope (d) and intercept of the plots of Tb vs 1/(scan speed]
| Base/Nucleotide | d (nm) | Tinter (ms) | Possible Kon M-1s-1 |
|---|---|---|---|
| dAmp | 0.32±0.002 | -0.34±1.2 | - |
| dCMP | 0.30±0.006 | 4.2±3.2 | 1.2 |
| dGMP | 0.30±0.002 | 0.61±0.9 | 8.2 |
| dTMP | 0.29±0.008 | 4.9±4.2 | 1.0 |
| dmeCMP | 0.3±0.015 | 5.2±7.5 | 5.0 |
| A in d(AAAAA) | 0.27±0.02 | -16±10 | - |
| C in d(CCCCC) | 0.24±0.03 | -18±18 | - |
