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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 15;189(2):1014–1023. doi: 10.4049/jimmunol.1200857

Figure 1. Low dose LPS selectively induces mild and prolonged expression of pro-inflammatory mediators.

Figure 1

(A) WT BMDM were treated with either a low dose LPS (50 pg/mL) or a high dose LPS (100 ng/mL) for the specified time periods. Total RNA was isolated, and real-time RT-PCR assays were performed to determine the expression levels of pro-inflammatory mediators such as Il-6 and Tnfα. (B) WT BMDM cells were treated with either a low dose LPS (50 pg/mL) or a high dose LPS (200 ng/mL) for the specified time periods. The levels of anti-inflammatory Il-10 and Ccl22 were measured by real-time RT-PCR. The relative transcript levels were standardized against Gapdh levels. Data were representative of at least three independent experiments. * p < 0.05.