Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Growth Horm IGF Res. 2012 Apr 30;22(0):108–115. doi: 10.1016/j.ghir.2012.04.001

Figure 1. InsR PCR primer design and standard plasmid template construction.

Figure 1

Figure 1

InsR-A and B isoform templates were synthesized and cloned into pUCminusMCS plasmid vectors. (A) InsR-A qPCR primers amplify a 50 bp region within the 404 bp encoded by exons 10 and 12. (B) InsR-B qPCR primers amplify a 42 bp region of the 440 bp encoded by exons 10, 11, and 12. A 20 bp ladder is shown.

HHS Vulnerability Disclosure