Figure 4.
17-HDHA decreases IL-6 and IL-10 production, but not TNFα. Positively selected CD19+ human B cells (3 × 106 cells/ml) were treated with 17-HDHA or vehicle 30 minutes prior to B cell stimulation with CpG ODN 2395 (1 μg/ml) plus αIgM (2 μg/ml). A) IL-10 concentrations measured by ELISA at day 7. B, C) IL-6 and TNFα concentrations measured by ELISA at day 2. Data were analyzed by one-way ANOVA with a Tukey’s post test, *p≤0.05, **p≤0.01 and represented as ±SEM.