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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 18;189(2):1036–1042. doi: 10.4049/jimmunol.1103483

Figure 6.

Figure 6

Antibody-secreting B cell differentiation is enhanced by 17-HDHA. Purified human B cells were collected on day 5 after activation. Number of antibody secreting cells was measured by ELISpot analysis. A) Representative image of IgM and IgG specific spots. B) Quantification of IgM and IgG spot forming cells. 7 days after activation, cells were harvested and stained for surface markers. A live-lymphocyte gate was used in all flow cytometry analyses as well as FMO for gating controls. C) Representative dot plot of double positive cells. D) Quantification of live IgD−CD27+CD38+ cells. Data analyzed by one-way ANOVA with Tukey’s post test, *p≤0.05, **p≤0.01 and represented as ±SEM.