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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 8;189(2):832–840. doi: 10.4049/jimmunol.1200854

Figure 2. Twist1 regulates Il12rb2 expression and STAT4 activation.

Figure 2

A, Naïve WT T cells were stimulated and transduced as described in Figure 1. Transduced Th1 cells were stimulated with IL-12 for 4 h and GFP+ cells were analyzed for phosphorylated STAT4 (pSTAT4). B, Phospho-STAT4 were determined in control or shRNA transduced Th1 cells following sorting for GFP expression. (C–D) Total cell extracts were immunoblotted for STAT4 and β-Actin as a control (C), and densitometry analysis of STAT4 protein is indicated (D). (E–F) Stat4 and Il12rb2 gene expression in Twist1 (E) or shTwist1 (F) transduced Th1 cells sorted for GFP expression. Data are mean of three to four independent experiments ± S.D. (A, B) or the average of replicate samples ± S.D. and representative of two to three independent experiments with similar results (C–F). *p<0.05