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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 6;189(2):711–720. doi: 10.4049/jimmunol.1102973

Figure 4. Innate immune responses induce autoantibodies in mice lacking IL-6, CD40L, and TNFα (3XKO).

Figure 4

(A) Representative IgM (red) and IgG (green) Hep-2 staining from B6 (top 2 rows) and 3XKO (bottom 2 rows), with (rows 2 and 4) and without (rows 1 and 3) LPS (1.25mg/kg days 0, 7, and 14). Images were captured at 60X magnification. (B) Compilation of fluorescence intensity of IgM autoantibodies from LPS-treated B6 (black) and 3XKO (white) mice. n=10 (C) Compilation of fluorescence intensity of IgG autoantibodies from LPS-treated B6 (black) and 3XKO (white) mice. n=10 (D) Total anti-nucleosome (IgG, IgM, IgA) levels from LPS-treated (1.25 mg/kg) B6 (black) and 3XKO (white) mice were quantitated on the indicated days. n= 7. (E) Total anti-nucleosome (IgG, IgM, IgA) levels from LPS-treated (0.9 mg/kg) B6 (black) and 3XKO (white) mice were quantitated on the indicated days. n= 5. Error bars depict SEM. **p≤0.01, * p≤0.05, NS; not significant, p>0.05).