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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 20;189(2):1081–1093. doi: 10.4049/jimmunol.1200318

Figure 10. IL-21R deficiency on host B cells decreases GCs in Bm12→B6 cGVHD.

Figure 10

Bm12→B6 cGVHD was induced as described in Materials and Methods. Recipient mice were sacrificed at the times indicated. A-F. Spleen sections from Bm12→B6 (A-C) and Bm12→IL-21R-/- (D-F) cGVHD mice were stained for B220 (blue) and PNA (brown). Original magnification x 200. G. Flow cytometry graphic analysis and contour plots of GL-7+ Fas+ GC cells gated on B220+ lymphocytes. H. Quantification of proliferating Ki-67+ GC B cells and representative flow cytometry profiles. I. RT-PCR for Aicda in purified B cells. mRNA levels were normalized to 18S and reported as fold increase over normal control. Data are representative of two independent experiments; n=5 mice/group. *=p<0.05