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. 2012 Mar 27;3(6):1249–1254. doi: 10.3892/ol.2012.658

Figure 1.

Figure 1

Anoikis of suspended SMMC-7721 cells and formation of multi-cellular spheroids. (A) Morphology of SMMC-7721 cells in agar suspension culture at indicated time-points (magnification, ×100). (B) Characterization of the suspended spheroids during suspension culture of SMMC-7721 cells. The amount of spheroids (3 cells/spheroid) per focus was counted under a phase-contrast microscope. The volume of spheroids referred to the average number of cells per multi-cellular spheroids (magnification, ×100; n=8–10). Each value is the mean ± SD of at least triplicate determinations. (C) Double staining of Hoechst 33342 and PI SMMC-7721 cells after suspension at different time-points by immunofluorescence under a laser scanning microscope (magnification, ×400).