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. 2012 Mar 27;3(6):1249–1254. doi: 10.3892/ol.2012.658

Figure 2.

Figure 2

CD147 was upregulated on the cell-cell contacts in suspended multi-cellular spheroids. (A) Western blotting of CD147 expression in SMMC-7721 cells following suspension at the indicated time-points. Two major forms of CD147 (45–66 and 35 kDa) were analyzed. Tubulin was used as a loading control. (B) Expression levels of CD147 in SMMC-7721 and HEK293ar cells following suspension at the indicated time-points as revealed by immunofluorescence under a laser scanning microscope (n=6-10; magnification, ×400). Yellow arrow points to the enhanced expression levels of CD147 between cell-cell contacts in a multi-cellular spheroid.