Skip to main content
. Author manuscript; available in PMC: 2013 Jul 6.
Published in final edited form as: Cell Stem Cell. 2012 Jul 6;11(1):50–61. doi: 10.1016/j.stem.2012.04.009

Figure 2. KDM6B is required for osteogenic differentiation of MSCs.

Figure 2

(A) Over-expression of KDM6B in MSCs. (B) Over-expression of KDM6B enhanced ALP activities in MSC differentiation. Values are mean ± s.d for triplicate samples from a representative experiment. **P < 0.01. (C) The over-expression of KDM6B enhanced mineralization in MSC differentiation. Mineralization was stained with Alizarin Red and qualitatively measured. **P < 0.01. (D) The knock-down of KDM6B by shRNA. MSC/KDM6Bsh, MSCs expressing KDM6B shRNA; MSC/Scrsh, MSCs expressing scramble shRNA. (E) The knock-down of KDM6B inhibited ALP activities in MSCs. Values are mean ± s.d for triplicate samples from a representative experiment. **P < 0.01. (F) The knock-down of KDM6B inhibited mineralization in MSCs. **P < 0.01. (G) The knock-down of KDM6B inhibited BSP expression in MSCs as determined by Real-time RT-PCR. **P < 0.01. (H) The knock-down of KDM6B inhibited OPN expression in MSCs as determined by Real-time RT-PCR. **P < 0.01. (I) The knock-down of KDM6B inhibited OCN expression in MSCs as determined by Real-time RT-PCR. **P < 0.01. (J) The restoration of KDM6B and mutant KDM6B in Kdm6b knock-down MSCs by Western blot analysis. (K) The catalytically mutant KDM6B could not restore the expression of osteoblast marker genes in MSCs. (L) The catalytically mutant KDM6B could not restore ALP activities in MSCs. (M) The knock-down of KDM6B inhibited BMP-enhanced ALP activities in MSCs. **P < 0.01. (N) The knock-down of KDM6B inhibited BMP-enhanced mineralization in MSCs. **P < 0.01. See also Figure S2