FIG. 5.

SASTG vectors exhibit reduced caspase-3/7 activation compared with adenoviral vectors in RNNCs. RNNCs were infected with Ad-luciferase (100 particles per cell) or SASTG-luciferase (50,000 particles per cell). At 24, 48, and 72 hr postinfection, equivalent protein amounts were used to assess luciferase activity (A), using the Promega luciferase assay reagent, or (B) caspase-3/7 activity, using the Promega Caspase-Glo 3/7 assay system. Both assays were analyzed with a Veritas luminometer from Turner Biosystems. (C) The ratio of vector-expressed luciferase to caspase-3/7 activity is shown. Each time point was examined in triplicate. Data represent means±SD.