Table 2.
eGFP Copy Numbers in Recipient Mice
| Donor | sTx recipient | # copies/dGE | Average |
|---|---|---|---|
| 1 | 1a | 0.01 | 0.01 |
| 1b | 0.01 | ||
| 1c | 0.01 | ||
| 2 | 2a | 0.00 | 0.006 |
| 2b | 0.01 | ||
| 2c | 0.01 | ||
| Total | 0.008 | ||
| Random integration | 0.008=0.8% |
dGE, diploid genome equivalent; sTx, serial transplant. After neonatal co-injection of 1×1010 vg of rAAV2/8-Fah and rAAV2/8-LSP-eGFP and 7 days of vanillin treatment, FAH+ hepatocytes were selected for and then serially transplanted to remove episomes. Data represent the number of copies of eGFP per diploid genome equivalent in serial transplant recipients. Roughly 50% of the hepatocytes in repopulated recipients were donor derived at the time of harvest, so the frequency was corrected by a factor of 2 to give an estimated random integration frequency of 0.8%.