FIG. 2.

NaHS protected against MPP⁺-induced cytotoxicity in primary cultured mesencephalic neurons from wild-type and Kir6.2^−/− mice. (a) Typical photograph of immunocytochemical staining showing THir neurons at ×100 magnifications. (b, c) Effects of NaHS on the number of TH⁺ neurons (b) and LDH release (c) in MPP⁺-treated DA neurons. (d) Typical western blotting revealed that NaHS alleviated ERS and apoptosis in primary mesencephalic neurons isolated from wild-type and Kir6.2^−/− mice. Statistical analysis of GRP78 (e), caspase 12 (f), and p65/H₃ (g) protein levels in neurons. Data are presented as the mean±S.E.M. of four individual experiments. **p<0.01 and *p<0.05 versus control group; ^##p<0.01 and ^#p<0.05 versus corresponding MPP⁺-treated groups; ^$p<0.05 versus Kir6.2^−/− group. ERS, endoplasmic reticulum stress; GRP, glucose-regulated protein; LDH, lactate dehydrogenase; MPP⁺, 1-methyl-4-phenylpyridinium. (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars).