Fig. 5.
Brat suppresses reversion of immature INPs by antagonizing Klu. (A-C) Removal of klu function suppresses supernumerary type II neuroblasts and restores the formation of INPs and GMCs in brat strong hypomorphic mutant brains. (A-B⁗) brat11/k06028 mutant Drosophila larvae carrying GFP-marked control (klu+/+) and klu mutant type II neuroblast mosaic clones (outlined by the yellow dotted line) were aged for 72 hours after clone induction and brains were stained for the markers indicated. (C) Quantification of various cell types in the control and klu mutant clones in brat11/k06028 mutant brains. (D-F) Co-expression of Brat suppresses Klu-induced supernumerary type II neuroblasts. (D-E⁗) Larvae carrying GFP-marked type II neuroblast lineage clones (outlined by the yellow dotted line) overexpressing klu or klu and brat were aged for 72 hours after clone induction and brains were stained for the markers indicated. (F) Average type II neuroblasts per brain lobe in larvae of the genotype indicated. Error bars indicate s.e.m. Arrows/arrowheads as Fig. 1. Scale bars: 10 μm.