Fig. 6.
Aberrant activation of Notch signaling induces reversion of immature INPs through klu. (A-D) Removal of klu function suppresses supernumerary type II neuroblasts induced by constitutively activated Notch signaling. (A-C⁗) Drosophila larvae carrying GFP-marked wild-type (klu+/+) or klu−/− type II neuroblast mosaic clones (outlined by the yellow dotted line) overexpressing Notchintra were aged for 72 hours after clone induction and brains were stained for the markers indicated. (D) The frequency of clones containing one or more type II neuroblasts in larvae of the genotype indicated. (E-G) Overexpression of klu prevents Notch mutant type II neuroblasts from premature differentiation. (E-F⁗) Larvae carrying GFP-marked Notch mutant type II neuroblast mosaic clones (outlined by the yellow dotted line) alone or overexpressing klu were aged for 72 hours after clone induction and brains were stained for the markers indicated. (G) The frequency of clones containing one or no type II neuroblasts in larvae of the genotype indicated. (H) Model: Brat or Numb prevent the reversion of immature INPs to type II neuroblasts by antagonizing Klu. Abbreviations and arrows/arrowheads as Fig. 1. Scale bars: 10 μm.