Fig. 6.

K box miRNA sponges enhance Notch signaling during wing development. (A,C-H) Notch[55e11]/+ (N/+) heterozygous females that carry ptc-Gal4 and two copies of the indicated miRNA sponges (SP); scr, scrambled sponge control. (A) N/+ females expressing control sponges exhibit a notch (asterisk) at the distal tip and mild vein thickening; the regions outlined are magnified to highlight these phenotypes. (B) Misexpression of any of the sponges used in this figure did not alter wing development; ptc-Gal4>2xmir-13aSP is shown as an example. The magnified insets exhibit normal vein thickness and can be used to judge N/+ haploinsufficiency. (C) Misexpression of the miR-13aSP rescued N/+ notching, but not vein thickening. (D) Misexpression of miR-7SP did not rescue either N/+ phenotype. Asterisk indicates area of wing notching. (E-H) Magnifications of the distal wing tips to highlight the status of wing notching in other sponge backgrounds. Asterisk indicates area of wing notching. (E) miR-2bSP, (F) miR-2cSP and (G) miR-13bSP all rescued N/+ notching, but (H) miR-6SP could not (asterisk). (I) Quantification of rescue of wing notching in various genotypes. N/+ in various ptc-Gal4>UAS-mir-SP backgrounds exhibit notching in about two-thirds of animals, this is reduced to less than 20% in the presence of miR-2cSP, to 10% in miR-2bSP and miR-13aSP, and to less than 2% in miR-13bSP. Inset shows the sequence relationship of these K box miRNAs.