Table 2.
Mouse PAb reactions against DENV-2 NS1 glycoprotein peptides prepared in the natural and reverse orientations
|
Peptide/Epitopea |
N/Rb |
Sequencec |
Average ELISA absorbance (492 nm) of PAbse |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| |
|
|
C57BL or B10 mouse haplotype anti-DENV-2 NS1glycoprotein PAbsd |
|||||||
| C57 | S | RIII | G | BR | D2N | A | S(C) | |||
| N-TERM |
+ |
5-VVSWKNKELKC-15 |
0.87 |
0.92 |
0.78 |
0.71 |
0.67 |
0.70 |
0.68 |
0.18 |
| N-TERM |
- |
15-CKLEKNKWSVV-5 |
0.56 |
0.58 |
0.54 |
0.56 |
0.43 |
0.51 |
0.55 |
0.14 |
| LD2 |
+ |
25-VHTWTEQYK-33 |
0.67 |
0.54 |
1.21 |
1.32 |
0.69 |
0.72 |
1.02 |
0.27 |
| LD2 |
- |
33-KYQETWTHV-25 |
0.44 |
0.37 |
0.83 |
1.24 |
0.26 |
0.45 |
0.77 |
0.25 |
| 24A |
+ |
61-TRLENLMWK-69 |
0.80 |
0.77 |
0.79 |
0.56 |
0.43 |
0.45 |
0.46 |
0.28 |
| 24A |
- |
69-KWMLNELRT-61 |
0.35 |
0.32 |
0.52 |
0.42 |
0.41 |
0.28 |
0.40 |
0.25 |
| N'LX1 |
+ |
109-TELRYSWKT-117 |
1.23 |
1.41 |
1.01 |
0.97 |
1.14 |
1.16 |
0.96 |
0.27 |
| N'LX1 |
- |
117-TKWSYRLET-109 |
0.98 |
0.86 |
0.82 |
0.88 |
0.99 |
1.06 |
0.89 |
0.22 |
| LX1 |
+ |
113-YSWKTWGKA-121 |
0.87 |
1.08 |
0.44 |
0.23 |
0.30 |
1.16 |
0.21 |
0.13 |
| LX1 |
- |
121-AKGWTKWSY-113 |
0.28 |
0.39 |
0.63 |
0.42 |
0.34 |
0.34 |
0.36 |
0.18 |
| LX2/1 |
+ |
209-TWKIEKASF-217 |
0.72 |
0.68 |
1.04 |
1.01 |
0.72 |
0.50 |
0.68 |
0.20 |
| LX2/1 |
- |
217-FSAKEIKWT-209 |
0.81 |
1.20 |
1.15 |
1.03 |
1.14 |
0.65 |
0.61 |
0.24 |
| LX2/2 |
+ |
267-PWHLGKLEM-275 |
0.92 |
0.79 |
1.02 |
1.04 |
0.76 |
0.68 |
0.63 |
0.21 |
| LX2/2 |
- |
275-MELKGLHWP-267 |
0.98 |
1.03 |
1.06 |
1.12 |
0.82 |
0.54 |
0.67 |
0.18 |
| 24C |
+ |
301-TTASGKLIT-309 |
0.65 |
0.74 |
0.61 |
0.66 |
0.47 |
0.40 |
0.43 |
0.13 |
| 24C |
- |
309-TILKGSATT-301 |
0.41 |
0.47 |
0.51 |
0.56 |
0.42 |
0.39 |
0.48 |
0.12 |
| LX2/3 |
+ |
331-YGMEIRPLK-339 |
0.65 |
0.71 |
1.24 |
1.01 |
0.69 |
0.81 |
0.62 |
0.26 |
| LX2/3 | - | 339-KLPRIEMGY-331 | 0.61 | 1.11 | 1.21 | 1.16 | 0.51 | 0.66 | 0.59 | 0.20 |
aName of the epitope or MAb 1G5.4-A1-C3 and/or 1C6.3 defined N’LX1, LX2/1, LX2/2 and LX2/3 peptide sequences that contained ELK/KLE-type or KELK/KLEK-type motifs.
bSynthetic peptide sequences prepared in either the natural (N: +) or reverse (R: -) orientations.
cSequence position and amino acid sequence of the DENV-2 NS1 glycoprotein represented by synthetic peptides prepared both the natural and reverse orientations. The ELK/KLE-type and KELK/KLEK-type motifs are underlined.
dGroups of C57BL (C57) (H-2b) or congeneic (H-2) B10 mouse strains (B10.S (S), B10.RIII (RIII), B10.BR (BR), B10.D2N (D2N) and B10.A (A)) immunized with the native hexameric DENV-2 e/sNS1 glycoprotein (gp), or the control protein (ovalbumin) (B10.S(C): (S(C)).
eAverage ELISA absorbance values of mouse PAb pools reacted with duplicate sets of synthetic peptides (standard deviation range: 0.00 (low values) to 0.04 (high values)). PAb absorbance (Abs) values which were ≥ 0.650 against the peptide prepared in the natural orientation that showed ≥ 1.50-fold or ≥ 2.50-fold RADS values (Abs against the natural (+)/reverse orientated peptide sequence), are underlined and shown in bold, respectively.