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. 2012 Jul 5;5:208. doi: 10.1186/1756-0500-5-208

Table 3.

Relative antibody discriminating specificity (RADS) values of MAbs and PAbs against the DENV-2 NS1 glycoprotein peptides

MAb/PAba Relative antibody discriminating specificity (RADS) valuec
Peptide/epitope on DENV-2 NS1 glycoprotein and sequence positionb
N-TM
 LD2
 24A
 N'LX1
 LX1
 LX2/1
 LX2/2
 24 C
 LX2/3
5-15 25-33 61-69 109-117 113-121 209-217 267-275 301-309 331-339
MAb
  
  
  
  
  
  
  
  
  
1H7.4
 *
 16.8
 *
 *
 *
 *
 *
 *
 *
5H4.3
 *
 *
 4.2
 *
 *
 *
 *
 *
 *
3D1.4
 *
 *
 *
 *
 15.0
 *
 *
 *
 *
1G5.3
 *
 *
 *
 *
 *
 *
 *
 12.4
 *
1C6.3
 1.12
 1.05
 0.47
 0.93
 *
 0.82
 1.12
 *
 1.67
1G5.4-A1-C3
 1.10
 0.88
 0.62
 1.26
 *
 *
 *
 *
 0.97
PAb
  
  
  
  
  
  
  
  
  
C57BL
 1.55
 1.52
 2.28
 1.26
 3.11
 0.89
 0.94
 1.59
 1.07
B10.S
 1.59
 *
 2.40
 1.64
 2.77
 0.57
 0.77
 1.58
 0.64
B10.RIII
 1.44
 1.46
 1.52
 1.24
 *
 0.90
 0.96
 *
 1.02
B10.G
 1.27
 1.07
 *
 1.10
 *
 0.98
 0.93
 1.18
 0.87
B10.BR
 1.56
 2.65
 *
 1.15
 *
 0.63
 0.93
 *
 1.35
B10.D2N
 1.37
 1.33
 *
 1.09
 *
 *
 1.26
 *
 1.23
B10.A 1.24 1.33 * * 1.08 1.11 * * *

aMonoclonal antibody (MAb) name or polyclonal antibody (PAb) pools from congeneic mouse strains (C57BL, B10.S, B10.RIII, B10.BR, B10.D2N and B10.A) generated against the DENV-2 NS1 glycoprotein.

bName of epitope defined by MAb 1H7.4, 5H4.3, 3D1.4 or 1G5.3 or peptide name identified by MAb 1G5.4-A1-C3 and/or 1C6.3 through their reactions with their ELK/KLE-type or KELK/KLEK-type motifs on the DENV-2 NS1 glycoprotein with their amino acid sequence location (N-TM = peptide N-TERM).

cRelative antibody discriminating specificity (RADS) value, determined from the average ELISA absorbance values of the MAbs or mouse PAb pools against synthetic peptides prepared in the natural/reverse orientations (fold difference of absorbance against the natural orientated peptide sequence/reverse orientated peptide sequence). MAb and PAb RADS values were only determined when they had absorbance (Abs) values of ≥ 0.650 against the peptide prepared in the natural orientation. RADS values of ≥ 1.50-fold or ≥ 2.50-fold are underlined and shown in bold, respectively. Reactions that were Abs < 0.650 against the peptide sequence prepared in the natural orientation are shown by asterixes.