Table 2.
Expressed genes during soybean (PI 595099) root and M. javanica- interaction.
| Contig | Blast best hit | Organism | Accession code | E-value | Number of ESTs PIN1 | Number of ESTs PII2 | Total number of ESTs |
|---|---|---|---|---|---|---|---|
| Contig1 | Auxin-repressed protein-like protein, Positives = 48/72 (66%) | Nicotiana tabacum | AY183722.1 | 1e-11 | 2 | 12 | 14 |
| Contig2 | Nuclear transport factor 2 (NTF-2), Positives = 113/124 (91%) | Arabidopsis thaliana | sp|Q9C7F5.1|NTF2_ARATH | 7e-57 | 3 | 10 | 13 |
| Contig3 | Metallothionein-like protein 2 (MT-2) | Cicer arietinum | sp|Q39459.2|MT2_CICAR | 5e-15 | 1 | 11 | 12 |
| Contig4 | Thioredoxin-like 4, Positives = 77/104 (74%) | Arabidopsis thaliana | sp|Q8LDI5.2|TRXL4_ARATH | 4e-27 | 5 | 6 | 11 |
| Contig5 | 60S ribosomal protein L23,Positives = 92/93 (98%) | Zea mays | gb|ACG48540.1| | 5e-69 | 3 | 5 | 8 |
| Contig6 | SLAH4 (SLAC1 HOMOLOGUE 4) Positives = 134/176 (76%) | Arabidopsis thaliana | ref|NP_001077757.1| | 5e-49 | 0 | 7 | 7 |
| Contig8 | SLAH1 (SLAC1 HOMOLOGUE 1); transporter Positives = 79/113 (69%), | Arabidopsis thaliana | ref|NP_176418.2| | 2e-29 | 0 | 6 | 6 |
| Contig9 | Zinc finger A20 and AN1 domain-containing stress-associated protein 8 (OsSAP8) Positives = 127/169 (75%) | Oryza sativa | sp|A2YEZ6.2|SAP8_ORYSI | 8e-56 | 0 | 5 | 5 |
| Contig10 | ACL098Cp, Positives = 28/59 (47%) | Ashbya gossypii | ref|NP_983306.1| | 2.7 | 2 | 3 | 5 |
| Contig11 | N-methyltransferase Positives = 133/160 (83%) | Arabidopsis thaliana | ref|NP_565246.1| | 8e-70 | 0 | 4 | 4 |
| Contig13 | Ubiquitin-conjugating enzyme E2-17 kDa 8 Positives = 148/148 (100%) | Arabidopsis thaliana | 834173 UBC8 | 2e-82 | 0 | 4 | 4 |
| Contig14 | Probable glutathione S-transferase (Heat shock protein 26A) (G2-4), Positives = 56/57 (98%) | Glycine max | gb|AAG34798.1|AF243363_1 | 2e-25 | 0 | 4 | 4 |
| Contig16 | Auxin-repressed protein Positives = 69/131 (52%) | Zea mays | gb|ACG37064.1| | 5e-13 | 1 | 5 | 6 |
| CL1Contig2 | |||||||
| Contig17 | Probableaquaporin PIP-type 7a (Turgor-responsiveprotein 7ª, 31), Positives = 107/108 (99%) | Medicago truncatula | gb|AAK66766.1|AF386739_1 | 1e-54 | 0 | 3 | 3 |
| Contig18 | Histone-lysine N-methyltransferase ASHR1, Positives = 30/54 (55%) | Arabidopsis thaliana | sp|Q7XJS0.2|ASHR1_ARATH | 6.1 | 1 | 2 | 3 |
| Contig19 | Auxin response factor 2 (ARF1-binding protein) (ARF1-BP) Positives = 49/57 (85%). | Lycopersiconesculentum | gb|ABC69711.1| | 9e-16 | 0 | 3 | 3 |
| Contig20 | Adenine phosphoribosyl transferase 1 (APRT 1), Positives = 164/174 (94%) | Solanum tuberosum | gb|ABB86271.1| | 2e-81 | 0 | 3 | 3 |
| Contig21 | Acetyl-CoAcarboxylase, alpha subunit Positives = 31/56 (55%) | Flavobacterium sp. MED217 | ref|ZP_01059904.1| | 34.7 | 0 | 3 | 3 |
| Contig25 | Chloroplast 50S ribosomalprotein L14, Positives = 94/94 (100%). | Glycine max | ref|YP_538801.1| | 5e-45 | 0 | 3 | 3 |
| Contig33 | Acyl carrier protein, mitochondrial precursor (ACP) (NADH-ubiquinoneoxidoreductase 9.6 kDasubunit) (MtACP-1), Positives = 79/120 (65%) | Arabidopsis thaliana | sp|P53665.1|ACPM_ARATH | ||||
| 9e-25 | 0 | 2 | 2 | ||||
| Contig34 | Kinesin light chain 3 (Kinesin light chain KLCt) Positives = 122/137 (89%) | Arabidopsis thaliana | gb|AAM63491.1| | 1e-54 | 0 | 2 | 2 |
| Contig35 | Predicted protein Positives = 30/57 (52%) | Pichia guilliermondii | gb|EDK41815.2| | 0.90 | 0 | 2 | 2 |
| Contig36 | Putative non-LTR retroelement reverse transcriptase, related Positives = 49/115 (42%) | Medicago truncatula | gb|ABN08132.1| | 1e-06 | 0 | 2 | 2 |
| Contig37 | ATP synthase 6 kDa subunit, mitochondrial Positives = 20/23 (86%) | Solanum tuberosum | sp|P80497.1|ATPY_SOLTU | 4e-05 | 0 | 2 | 2 |
| Contig38 | Transcription factor MYBZ2 Positives = 131/131 (100%) | Glycine max | gb|ABI73970.1| | 6e-119 | 0 | 2 | 2 |
| Contig39 | Thioredoxin-like protein 1 Positives = 149/169 (88%) | Zea mays | gb|ACG24478.1| | 1e-66 | 0 | 2 | 2 |
| Contig41 | No hit blast | 0 | 2 | 2 | |||
| Contig42 | Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit DAD1 (Defender against cell death 1) (DAD-1) (AtDAD1), Positives =111/113 (98%), | Arabidopsis thaliana | ref|NP_174500.1| | 3e-52 | 0 | 2 | 2 |
| Contig43 | Anaphase-promoting complex subunit 11 (APC11) (Cyclosome subunit 11), Positives = 67/83 (80%) | Mus musculus | sp|Q9CPX9.1|APC11_MOUSE | 2e-36 | 0 | 2 | 2 |
| Contig44 | Unnamed protein product Positives = 39/54 (72%) | Vitis vinifera | emb|CAO40176.1| | 5e-05 | 1 | 1 | 2 |
| Contig46 | Inner membrane magnesium transporter mrs2, mitochondrial precursor (RNA-splicing protein mrs2), Positives = 58/120 (48%), | Schizosaccharomyces pombe | sp|P87149.1|MRS2_SCHPO | 8e-05 | 0 | 2 | 2 |
| Contig48 | F-box/LRR-repeat protein 16 (F-box and leucine-rich repeat protein 16) Positives = 131/144 (90%) | Malus x domestica | gb|ACB87911.1| | 6e-57 | 0 | 2 | 2 |
| Contig50 | Glucose-6-phosphate 1-dehydrogenase, cytoplasmicisoform (G6PD) Positives = 89/93 (95%) | Solanum tuberosum | gb|ABB55386.1| | 1e-44 | 0 | 2 | 2 |
| Contig51 | Hypothetical protein MtrDRAFT_AC136139g5v2, Positives = 35/38 (92%). | Medicago truncatula | gb|ABE93033.1| | 8e-12 | 0 | 2 | 2 |
| Contig52 | USP family protein Positives = 27/30 (90%) | Zea mays | gb|ACG42306.1| | 6e-06 | 0 | 2 | 2 |
| Contig53 | Unnamed protein product Positives = 111/119 (93%) | Vitis vinifera | emb|CAO42347.1| | 6e-53 | 0 | 2 | 2 |
| Contig54 | 39S ribosomal protein L41-A, mitochondrial precursor Positives = 79/89 (88%) | Arabidopsis thaliana | ref|NP_568574.1| | 8e-35 | 0 | 2 | 2 |
| Contig57 | Chalconereductase Positives = 89/106 (83%) | Sesbania rostrata | emb|CAA11226.1| | 3e-33 | 0 | 2 | 2 |
| Contig58 | Probable rhamnose biosynthetic enzyme 1 Positives = 94/103 (91%) | Arabidopsis thaliana | sp|Q9SYM5.1|RHM1_ARATH | 7e-46 | 0 | 2 | 2 |
| Contig60 | Ubiquinol—cytochrome-c reductase-like protein Positives = 83/85 (97%) | Arabidopsis thaliana | dbj|BAD95225.1| | 5e-43 | 0 | 2 | 2 |
| Contig61 | Translation initiation factor IF-2 Positives = 39/80 (48%) | Plasmodium yoelii | ref|XP_730210.1| | 3.0 | 0 | 2 | 2 |
| Contig62 | UPF0497 membrane protein At2g28370, Positives = 49/55 (89%) | Arabidopsis thaliana | sp|Q9SKN3.1|U4977_ARATH | 1e-20 | 0 | 2 | 2 |
| Contig63 | MADS-box protein SOC1 (protein suppressor of constans overexpression 1) Positives = 77/82 (93%) | Glycine max | gb|ABC75835.1| | 3e-33 | 0 | 2 | 2 |
| Contig64 | SAP domain-containing protein Positives = 84/117 (71%) | Arabidopsis thaliana | ref|NP_201151.2| | 3e-22 | 0 | 2 | 2 |
| Contig65 | No blast hit | - | - | - | 0 | 2 | 2 |
| Contig66 | Histone H2A.F/Z Positives = 115/116 (99%) | Arabidopsis thaliana | emb|CAA73155.1| | 4e-54 | 0 | 2 | 2 |
| Contig68 | Hypothetical protein MGG_13574 Positives = 28/54 (51%) | Magnaporthe grisea | ref|XP_001408018.1| | 5.9 | 0 | 2 | 2 |
| Contig69 | No significant | - | - | - | 0 | 2 | 2 |
| Contig70 | Ferredoxin Positives = 102/118 (86%) | Zea mays | gb|ACG39554.1| | 4e-43 | 0 | 2 | 2 |
| Contig71 | HMG1/2-like protein (Protein SB11) Positives = 119/121 (98%) | Glycine max | sp|P26585.1|HMGL_SOYBN | 3e-33 | 0 | 2 | 2 |
| Contig72 | ABI5 binding protein A1 Positives = 47/54 (87%) | Triticum aestivum | dbj|BAG12827.1| | 3e-25 | 0 | 2 | 2 |
| Contig73 | NAC domain-containing protein 29 (ANAC029) (NAC2) Positives = 64/102 (62%) | Arabidopsis thaliana | sp|O49255.1|NAC29_ARATH | 2e-17 | 0 | 2 | 2 |
| Contig74 | At3g08610 Positives = 58/62 (93%) | Arabidopsis thaliana | gb|AAP21180.1| | 4e-23 | 0 | 2 | 2 |
| Contig77 | Metallo-beta-lactamase superfamily protein Positives = 48/105 (45%) | Alcanivorax sp. | gb|EDX88588.1| | 1.4 | 0 | 2 | 2 |
| Contig79 | 4-Coumarate—CoAligase-like 5 (Peroxisomal OPC-8:0-CoA ligase 1), Positives = 175/199 (87%). | Oryza sativa | sp|Q10S72.1|4CLL4_ORYSJ | 2e-87 | 0 | 2 | 2 |
| 55 | - | - | - | - | 19 | 170 | 189 |
1
PIN = PI 595099 uninoculated;
2
PII = PI 595099 inoculated with M. javanica.