Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jul 9;198(1):47–55. doi: 10.1083/jcb.201111088

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Fan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Myo1c is required for G-actin delivery to the leading edge and optimal cell migration. (A) Knockdown of Myo1c by shRNA and siRNA. Cells were transfected with shRNA targeted against Myo1c or Luc or Myo1c or scrambled siRNA, and Myo1c protein was detected by immunoblotting. GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (B) Cells were transfected with a plasmid coexpressing DsRed and shRNA targeted against Luc or Myo1c and stained to visualize G- or F-actin. The insets show coexpressed DsRed. Long arrows indicate the direction of cell migration. Filled arrows indicate regions enriched in G- or F-actin, and open arrows indicate the cell leading edge in regions of reduced G- or F-actin. CMV, cytomegalovirus. (C) Cells were cotransfected with vectors expressing paGFP-actin^G13R and shRNA targeted against Luc or Myo1c, and paGFP-actin^G13R movement was detected by photoactivation. Dynamics in the forward region near the leading edge fluorescence intensity after photoactivation in the lamellipodia are shown. (inset) Mean fluorescence intensity (MFI) 15 s after photoactivation (mean fluorescence intensity ± SEM; n = 8–12 cells). (D) Cells were transfected with Myo1c or scrambled siRNA and subjected to wound-induced migration and VEGF-A–induced chemotaxis. The number of migrating cells was determined (mean ± SEM; three independent experiments).