Figure 1.

Rescue experiments demonstrate that CG11727 requires its GAP activity in vivo. (A) Schematic representation of Drosophila egg chambers of stage 9 and 10. Red represents the actin staining, blue represents the nucleus, and green represents the BCs. BCs migrate from the anterior tip toward the posterior oocyte, left to the right in all images. Arrows point the BC clusters. Asterisks mark the oocytes. (B) Schematic representation of the Evi5 gene and its human orthologues EVI5 and EVI5-like. The sequence similarity in the TBC domain between Evi5 and its human orthologue and the proposed targets of EVI5 and EVI5-like are depicted. The position of the catalytic arginine residue in Evi5 mutated for an alanine is illustrated. (C–F) Representative examples of BC migration in a stage 10 egg chamber under the indicated conditions using the C306-Gal4 driver. Control (C) or RNAi(Evi5) (D) is represented. Representative examples of BCs expressing RNAi(Evi5) and in which Evi5 was reintroduced by expression of Evi5-mcherry (E) or Evi5^RA-mcherry (F). BCs are stained with phalloidin (red) and DAPI (blue). Arrows point to BC clusters. Bar, 20 µm. (G) The percentage of migration is expressed as the migration index (M.I.) and completion index (C.I.) for the indicated conditions above (35 < n < 161). Error bars are standard error of the mean.