Table 1.

M.I and C.I. resulting from knockdown of every potential Rab-GAP protein during BC migration

Drosophila identifier	Common name	dsRNA (VDRC no.)	M.I.	C.I.	n	Human orthologue
CG1093	Pollux	27335	N/A	N/A	N/A	TBC1D1 and TBC1D4
		106969	0.98	0.95	20
CG1695	CG1695	20340	0.88	0.69	29	SGSM1-2
		48062	0.98	0.95	22
		106947	0.875	0.74	34
CG4041	CG4041	34780	0.83	0.67	3	TBCK
		108887	0.98	0.95	55
CG4552	CG4552	40538	0.51	0.30	41	TBC1D23
		110700	0.98	0.96	47
CG5337	CG5337	22070	0.99	0.94	17	TBC1D16
		22069	0.96	0.90	20
		110067	1.00	1.00	20
CG5344	Whacked	22081	0.96	0.92	24	TBC1D10A-B-C
		22082	0.88	0.75	44
CG5745	CG5745	35034	0.97	0.93	45	TBC1D22A-B
		35036	0.94	0.84	32
		108659	1.00	1.00	21
CG5916	CG5916	110561	0.95	0.90	20	TBC1D6
CG5978	CG5978	21000	0.98	0.94	72	TBC1D13
		21001	0.97	0.92	38
		110396	0.97	0.93	28
CG6182	CG6182	14705	0.88	0.78	32	TBC1D7
		14706	0.97	0.93	15
		106667	0.98	0.94	34
CG7061a	CG7061	27823	0.97	0.94	17	RAB3GAP2
		27824	0.88	0.79	39
		106905	0.54	0.41	39
CG7112	GapcenAb	35174	0.92	0.82	91	RAB-GAP1(GAPCENA) and TBC1D18
		103588	0.97	0.93	61
CG7324	CG7324	32929d	0.96	0.89	232	TBC1D8B-9B
CG7742	CG7742	25535	1.00	1.00	16	TBC1D19
		25536	0.99	0.97	32
		100125	0.99	0.96	28
CG8085	RN-tre	28192ce	0.56	0.36	96	RNTRE (USP6NL), TBC1D28, USP6, TBC1D3F-G-H
		28194c	0.65	0.42	73
		108670	0.87	0.75	60
CG8155	CG8155	24218	0.85	0.75	28	TBC1D25
		24221	0.58	0.42	31
		108444	0.99	0.95	22
CG8449	CG8449	24102	1.00	1.00	17	TBC1D5
CG9339	CG9339	44655	0.99	0.98	42	TBC1D24
		108736	0.99	0.96	23
CG11490	Tbc1d15-17b	20040	0.95	0.90	66	TBC1D15-17
		1096668	0.94	0.84	51
CG11727	Evi5b	17548	0.76	0.61	85	EVI5 and EVI5L
		17549e	0.32	0.14	161
		105146	0.88	0.73	49
CG12241	CG12241	33729d	0.95	0.90	201	SGSM3
CG16896	CG16896	20315	0.82	0.76	17	WDR67
		20316	0.97	0.88	18
		107134	0.91	0.81	43
CG17883	CG17883	30277	0.94	0.83	30	TBC1D20
CG32506	CG32506	28776d	0.96	0.89	232	SGSM1-2
CG32580	CG32580	105591	0.99	0.96	24	MUC16
CG33715	Msp-300	25906	0.97	0.92	83	SYNE1-2 and CLMN
		40143	1.00	1.00	12
		40145	0.98	0.95	42
		50192	0.71	0.58	19
		107183	0.99	0.98	56
		109023	0.97	0.93	29
CG42795	CG42795	17314	0.95	0.90	73	TBC1D30
		108779	0.98	0.93	29

N/A, not applicable: this dsRNA line affects drastically the morphology of the egg chamber. VDRC, Vienna Drosophila RNAi Center.

^aThis Rab-GAP protein does not have a TBC domain.

^bThese common names have been introduced in this study.

^cCrosses were performed at 25°C to minimize morphological phenotypes.

^dLines obtained from TRiP at Harvard Medical School.

^eThis RNAi fly line is used subsequently is this study.