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. 2012 Jul 9;198(1):127–141. doi: 10.1083/jcb.201205025

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© 2012 Fontana et al.

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Figure 2. — Activation of expression upon nerve injury of neurotrophin genes by denervated SCs depends on Jun up-regulation. (A) Jun ISH on a longitudinal section of a wild-type mouse injured sciatic nerve, 7 d after injury. The approximate crush lesion is indicated by a transverse dotted line. Proximal and distal stump directions are also indicated. Rectangles mark two representative areas, depicted in B and C. (B and C) High magnification from A of the proximal (left) and distal (right) stumps to the lesion correspondingly. (D) Confocal imaging of 7 d post-injury wild-type (Jun^f/f) and mutant (Jun^ΔSC) longitudinal sciatic nerve sections. Double immunofluorescence for the SC progenitor marker p75 neurotrophin receptor (p75NTR; green, left) and total c-Jun (red, middle). Merge images are shown on the right. DNA (blue) was counterstained with DAPI. Arrows indicate p75NTR+ SC progenitors that coexpress c-Jun. (E) Superarray comparing the neurotrophin gene expression level of sciatic nerves 7 d after injury. Fold change values in the mutant nerves are displayed by normalizing to wild-type levels (baseline value 1; n = 7 for Jun^f/f mice and n = 6 for Jun^ΔSC mice from a single experiment). (F) Q-PCR confirmation of the results obtained with the superarray shown in E. Fold change values in the mutant nerves are displayed by normalizing to wild-type levels (baseline value 1; mean of three replicates, n = 7 for Jun^f/f mice and n = 6 for Jun^ΔSC from a single experiment). Bars: (A) 100 µm; (B; also applies to C) 25 µm; (D; bar in top left image applies to the whole panel) 10 µm.