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. 2012 Apr 10;20(7):1367–1377. doi: 10.1038/mt.2012.45

Figure 1.

Figure 1

Effects of the NFL-TBS.40-63 peptide on the microtubule cytoskeleton of rat glioma cells and primary astrocytes and neurons. Rat F98 glioma cells and rat primary astrocytes or neurons were grown in the presence of NFL-TBS.40-63 or NFL-SCR peptides (10 µmol/l) for 6 hours. Microtubules were detected using an antitubulin antibody (red), and the biotinylated NFL-TBS.40-63 peptide was detected using Alexa-labeled avidin (green). Cells were examined with a confocal microscope and numerous fluorescent aggregates, corresponding to the biotinylated peptide typically were observed. Glioma cells containing NFL-TBS.40-63 lack a normal microtubule network and typically assume a spherical shape. Except for rare cells demonstrating one or two relatively weakly labeled aggregates, primary rat astrocytes or neurons cultured under similar conditions remained unlabelled and their microtubule networks appeared unaltered. Incubation with the scrambled NFL-SCR peptide did not alter microtubule organization in astrocytes, neurons or glioma cells. White bars, 10 µm. NFL, neurofilament light; SCR, scrambled; TBS, tubulin-binding site.