Figure 3.

Peripheral immune system was not impacted by i.c.v. injection of shAct1 lentivirus. Splenocytes were harvested at the end point of the experiment shown in Figure 2b. (a) Total cell numbers were counted and (b) frequencies of IFN-γ⁺ and IL-17⁺ cells in gated CD4⁺ T cells were measured by flow cytometry. (c) After 3 days' culture, cytokine production in culture supernatants was determined by ELISA and (d) T cell proliferation determined by 3H-incorporation. Results are shown as mean ± SD (n = 5 each gro up). One representative of three experiments is shown. cpm, counts per minute; ELISA, enzyme-linked immunosorbent assay; i.c.v., intracerebroventricular; IFN, interferon; IL, interleukin, n.s., not significant.