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. 2012 Mar 20;20(7):1338–1348. doi: 10.1038/mt.2012.12

Figure 6.

Figure 6

Expression of chemokines, cytokines, and neurotrophic factors in the CNS after shAct1 treatment. Total RNA was prepared at the end of experiment from a fraction of spinal cords of shAct1-treated or control lentivirus-treated EAE mice described in Figure 2b. RT-PCR was performed to determine mRNA levels of (a) Act1, (b) receptors for IL-17 family cytokines, (c) cytokines, and (d) chemokines. (e) Expression of several Th1- and Th17-related chemokines and cytokines was confirmed by western blot. (f) Expression of iNOS, and (g) neurotrophic factors BDNF and NT-3 was determined by real-time PCR. Relative expression was calculated by −ΔΔCt values from triplicate PCR. *P < 0.05, **P < 0.01 (n = 5 each group). One representative of two experiments is shown. BDNF, brain-derived neurotrophic factor; CNS, central nervous system; CNTF, ciliary neurotrophic factor; EAE, ex perimental autoimmune encephalomyelitis; FGF2, fibroblast growth factor-2; GM-CSF, granulocyte-macrophage colony-stimulating factor; IGF1, insulin-like growth factor-1; IL, interleukin; iNOS, inducible nitric oxide synthase; mRNA, messenger RNA; n.s., not significant; NT-3, neurotrophin-3; RT-PCR, reverse transcription-PCR; TGF-β, transforming growth factor-β Th1, T-helper 1.