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. 2012 Feb 28;20(7):1384–1392. doi: 10.1038/mt.2012.34

Figure 7.

Figure 7

Injection of purified recombinant human collagen Q (ColQ)- tailed acetylcholinesterase (AChE). (a) Daily injection of 0.2 µg human recombinant ColQ-tailed AChE into the gluteus maximus muscles of Colq−/− mice rescues AChE activity and ColQ in the noninjected triceps where they are colocalized to acetylcholine receptors (AChR). Bar = 10 µm. (b) The size of ColQ-positive area is normalized for the size of AChR-positive area at the neuromuscular junctions (NMJs) of noninjected triceps. (c) Signal intensities of ColQ at the NMJs of noninjected triceps. Mean and SE are indicated. WT, wild-type mice, number of NMJs = 43; Protein injected, mice injected with ColQ-tailed AChE, number of NMJs = 42. *P < 0.001. Signal intensities are normalized to that of Colq−/− mice. Quantitative analyses were performed with the BZ-9000 microscope and the Dynamic Cell Count software BZ-H1C (Keyence).