Figure 2.

Quantification of myotube size and nuclear area using the Photoshop imaging method. Representative micrographs of a control myotube (A) and myotube cultured with 100 ng/ml rh-Wnt3A (B) are shown. Scale bar = 100 μm. (C) Individual data points for all measured myotubes in control and rh-Wnt3A conditions; horizontal red lines indicate the mean values. rh-Wnt3A–treated myotubes form myotubes over a larger size range than controls. Due to nuclear clustering, data were not presented on nuclear area/myotube (see text for discussion). Where significant clustering does not occur, the measurement of nuclear area using the proposed method would allow quantification of the two-dimensional myonuclear domain of these syncytia.