Fig. 1.

Western verification of periostin knockout mice. Protein lysates were generated from skin biopsies from wild-type (+/+), heterozygous (+/−), and periostin null mice (−/−), and subjected to Western analysis for periostin expression. Expression of periostin isoforms is seen around the predicted 90–100 kDa and the 37 kDa molecular weights. The heterozygotes decrease in total periostin expression by roughly half and no expression is detected in the periostin nulls. β-tubulin was used as a normalization control.