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. Author manuscript; available in PMC: 2012 Aug 10.
Published in final edited form as: Sci Transl Med. 2011 Aug 10;3(95):95ra73. doi: 10.1126/scitranslmed.3002842

Fig. 4.

Fig. 4

Prolonged surface CAR19 expression and establishment of functional memory CART19 cells in vivo. (A and B) T cell immuno-phenotyping of CD4+ (A) and CD8+ (B) T cell subsets. Frozen peripheral blood (PB) samples from UPN 03 obtained at days 56 and 169 after T cell infusion were subjected to multiparametric immunophenotyping for expression of markers of T cell memory, activation, and exhaustion; data are displayed after biexponential transformation for objective visualization of events. (C) Functional competence of persisting CAR cells. Frozen PB samples from UPN 03 obtained at days 56 and 169 after T cell infusion were evaluated directly ex vivo for the ability to recognize CD19-expressing target cells using CD107 degranulation assays. Presented data are for the CD8+ gated population. The gating strategies for these figures are presented in fig. S2.