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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Cell Signal. 2011 Nov 18;24(3):794–802. doi: 10.1016/j.cellsig.2011.11.014

Figure 5. The α1B-AR Forms Receptor Oligomers in Adult Cardiac Myocytes.

Figure 5

(A) Representative confocal images of α1B-WT (left) and α1B-NLSmut (right) in α1ABKO cardiac myocytes. (B) Representative confocal images of α1B-WT (left) and α1B-NLSmut (right) in α1AKO cardiac myocytes which express only endogenous α1B-ARs. (C) Representative confocal images of α1B-WT (left) and α1B-NLSmut (right) in α1BKO cardiac myocytes which express only endogenous α1A-ARs. Final magnification = 600x. (D) Comparison of the degree of nuclear localization of the α1B-NLSmut in α1ABKO, α1AKO, and α1BKO myocytes. The degree of nuclear localization was determined by scoring each image on a scale from 0 to 5 with higher values representing increased nuclear fluorescence. Cells with nuclear and non-nuclear were scored 0 = no nuclear fluorescence, 1 = up to 25% of the nuclear membrane was identifiable, 2 = 26–50% of the nuclear membrane was identifiable, 3 = 51–75% of the nuclear membrane was identifiable, 4 = 76–100% of the nuclear membrane was identifiable. A score of 5 was given to cardiac myocytes with nuclear fluorescence and no non-nuclear fluorescence. Scores from three blinded technicians were included in the statistical analysis (n=6 for each genotype).

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