Fig. 3.

Radiation stimulated dissociation of the beclin1-bcl2 complex and downregulated of Akt1-mTOR pathway in the lungs of K-ras^LA1 mice.

(a) SDS-PAGE of the beclin1 and bcl2 lung lysate immunoprecipitation. IP beads were bound to the beclin1 antibody and immunoblotted with the bcl2 antibody (1:500 dilution). (b) Western blot analysis using phospho-Akt1 at Thr308 (1:500 dilution), phospho-Akt1 at Ser473 (1:500 dilution) and Akt1 (1:5000 dilution). (c) Western blot analysis with phospho-mTOR at Ser2448, mTOR, raptor and rictor antibodies diluted 1:1000. Bands are representative of five individuals from each group. Representative figures of five mice per group.